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Image analysis for correction of electron microscopes
Smital, Petr ; Schwarz, Daniel (referee) ; Kolář, Radim (advisor)
This thesis describes the physical nature of corrections of an electron microscope and mathematical methods of image processing required for their complete automation. The corrections include different types of focusing, astigmatism correction, electron beam centring, and image stabilisation. The mathematical methods described in this thesis include various methods of measuring focus and astigmatism, with and without using the Fourier transform, edge detection, histogram operations, and image registration, i.e. detection of spatial transformations in images. This thesis includes detailed descriptions of the mathematical methods, their evaluation using an “offline” application, descriptions of the algorithms of their implementation into an actual electron microscope and results of their testing on the actual electron microscope, in the form of a video footage grabbed from its control computer’s screen.
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Retinal image registration using phase correlation
Prosser, Jan ; Harabiš, Vratislav (referee) ; Kolář, Radim (advisor)
This master’s thesis is aimed at registration of frames of retinal fundus video using phase corre- lation. An introduction describes general research in topic of retinal fundus, eye movements, diff erent approaches for image registration, phase correlation and examples of phase corre- lation applications. The second, practical part of master’s thesis, is dedicated to description of the proposed algorithm for registration of frames of retinal fundus video. The description of the proposed algorithm is divided into three parts. First two parts describe how frames of retinal fundus video are rated in terms of suitability for registration. Third part describes image registration algorithm itself. In conclusion, the accuracy of algorithm and computational time are evaluated.
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Retinal image registration using phase correlation
Prosser, Jan ; Harabiš, Vratislav (referee) ; Kolář, Radim (advisor)
This master’s thesis is aimed at registration of frames of retinal fundus video using phase corre- lation. An introduction describes general research in topic of retinal fundus, eye movements, diff erent approaches for image registration, phase correlation and examples of phase corre- lation applications. The second, practical part of master’s thesis, is dedicated to description of the proposed algorithm for registration of frames of retinal fundus video. The description of the proposed algorithm is divided into three parts. First two parts describe how frames of retinal fundus video are rated in terms of suitability for registration. Third part describes image registration algorithm itself. In conclusion, the accuracy of algorithm and computational time are evaluated.
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Image analysis for correction of electron microscopes
Smital, Petr ; Schwarz, Daniel (referee) ; Kolář, Radim (advisor)
This thesis describes the physical nature of corrections of an electron microscope and mathematical methods of image processing required for their complete automation. The corrections include different types of focusing, astigmatism correction, electron beam centring, and image stabilisation. The mathematical methods described in this thesis include various methods of measuring focus and astigmatism, with and without using the Fourier transform, edge detection, histogram operations, and image registration, i.e. detection of spatial transformations in images. This thesis includes detailed descriptions of the mathematical methods, their evaluation using an “offline” application, descriptions of the algorithms of their implementation into an actual electron microscope and results of their testing on the actual electron microscope, in the form of a video footage grabbed from its control computer’s screen.
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